To: Lesley Grofq : Richard Kahn From: jeffrey E. Sent Mon 6/23/2014 5:41:50 PM Subject: Re: Joe Thakuria plese send him my money today. On Mon, Jun 23, 2014 at 1:33 PM, Lesley Groff wrote: Please see below...do you want to send him the 4 other samples? Lesley, thanks for the email. Jeffrey, can add whoever he want into the YPO exome study but I will need to have those samples mailed to me directly and received by next Monday at the latest to be included in this sequencing run. The exomes (at cost) are $1000 each. I'll add this to his invoice which I'll send before the end of the week. I don't want this getting lost or held up in HMS or MGH interoffice mail. So, please mail to my home address: Joe Thakuria He should have (if I remember correctly) 4 extra kits. If he needs more, let me know right away because we'll be cutting it close in terms of getting into this round (with mailing it out to him and then back to me). The instructions in the pack are straightforward and he just did it recently but, to summarize: I. do not eat/drink/smoke or chew gum for 30minutes prior to collecting (eg, if you ate a chili dog, we could get contamination from pork, beef, chicken, tomato, kidney bean, and chili pepper genomes; you'd be surprised how much similarity there is on the DNA level between very different species) 2. spit into the funnel and fill to the indicated line (not all the way to the top so there's room for #3) and make sure this isn't filled with bubbles. If there are bubbles it may need to settle a little so the process typically takes 2 - 5 minutes. 3. There arc 2 caps. When done spitting, close the first cap (attached to the funnel the person spits into) so that you hear a click and the liquid in that first cap goes into the tube. That liquid needs to mix with the collected saliva. 4. Then take that first cap off an put the second smaller cap on and close tightly. Make sure the cap is on well and not leaking (or the sample can't b