From: Sultan Bin Sulayem To: Jeffrey Epstein [email protected]> Subject: Fuss hospital china pioneers cancer treatment Date: Tue, 08 Nov 2011 06:32:05 +0000 put In the keywords for Search IFearth I Number ol freeze-Maw cycles There is some evidence that a double freeze-than cycle induces a higher percentage of tissue elesbuction within a given lesion. thus improves uniformity of cell death. Kollman(20' using a porcine model. studied ?inuatiepatic cryolesons induced by freezing the hepatic tissue for a total of 15 mins. Additional animals underwent a double freeze-than cycle of 7.5 mins each (DF). Seven days after freeong. OF did not change the volume of the env:lesion compared to SF. however. resulted in enhanced destrudion of hepatocyle nuclear morphology. Ile showed that double freezing may improve uniformity of truancy* nuclear destruction wthn the margin of the lesion due lea more pronounced shutdown of microvascular perfusion. resultng in irevernble iv:herrn (21.22). In addition. as it is well known that thawing is a more Overlent mechanism of cell death than cooling (231. the application of a double thaw cycle in double freezing may account for he more complete marghal hepatocyte nuclear destruction. Rolinson(24] showed that for bone cryostspery. the difference of the viability of targeted cells between one freezing cycle and two freezing cycles was Nghly signficanL But the viability blowing three freezing cycles was similar to that alter two freezing cycles (Fore 18.10). Also. after a singe cycle of freezing, most specimens were necrotic. some were still viable. to contrast. there were no specimens that had viable cells following two freezing cycles (Figure 1-8.11). Figure 1-8-10. Average viability 8 standard deviation of tumor morsels (expressed as optical density per 100 mg wet weir t) From Rolinson7 ef W. Crycebioloa 44 0 Vw ; El.; A Figure 1-8-11.7 A. Cell recluses following a single freezing cycle is not atiform (Aldan blue s